منابع مشابه
Using ubiquitin to follow the metabolic fate of a protein.
We describe a method that can be used to produce equimolar amounts of two or more specific proteins in a cell. In this approach, termed the ubiquitin/protein/reference (UPR) technique, a reference protein and a protein of interest are synthesized as a polyprotein separated by a ubiquitin moiety. This tripartite fusion is cleaved, cotranslationally or nearly so, by ubiquitin-specific processing ...
متن کاملThe natural history of ubiquitin and ubiquitin-related domains.
The ubiquitin (Ub) system is centered on conjugation and deconjugation of Ub and Ub-like (Ubls) proteins by a system of ligases and peptidases, respectively. Ub/Ubls contain the beta-grasp fold, also found in numerous proteins with biochemically distinct roles unrelated to the conventional Ub-system. The beta-GF underwent an early radiation spawning at least seven clades prior to the divergence...
متن کاملUbiquitin and ubiquitin-like modifications of the p53 family.
Regulation of p53 by the ubiquitin-proteasomal pathway has been studied considerably. Studies have also demonstrated that the ubiquitin-like proteins SUMO-1 and NEDD8 modify p53. Similarly, p63 and p73 are subject to regulation by ubiquitin and ubiquitin-like modifications, and perturbations of these pathways in the regulation of the p53 family have been implicated in tumorigenesis and developm...
متن کاملStructure and ubiquitin binding of the ubiquitin-interacting motif.
Ubiquitylation is used to target proteins into a large number of different biological processes including proteasomal degradation, endocytosis, virus budding, and vacuolar protein sorting (Vps). Ubiquitylated proteins are typically recognized using one of several different conserved ubiquitin binding modules. Here, we report the crystal structure and ubiquitin binding properties of one such mod...
متن کاملEngineering ubiquitin to modulate the ubiquitin proteosome system.
Ubiquitination determines the longevity of proteins in cells and plays major roles in signaling. The complexity of the ubiquitination proteasome system (UPS) is evident in the number of enzymes that are involved, including hundreds of ligases and roughly a hundred deubiquitinases (DUBs) that add or remove ubiquitin chains, respectively. Furthermore, ubiquitin modifications on proteins are recog...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2008
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0508-380